Paracrine Regulation of Steroidogenesis in Theca Cells by Granulosa Cells Derived from Mouse Preantral Follicles

Figure 2

Effect of GCs on steroidogenesis in TCs. Concentrations of testosterone and androstenedione in the spent medium were measured by radioimmunoassay and enzyme linked immunosorbent assay, respectively. (a) and (c) An increased amount of testosterone and androstenedione was observed in cocultured model. (b) and (d) GCs produced diminished levels of testosterone and androstenedione, respectively. Note the scale difference between (a) and (c) as well as between (b) and (d). Data were expressed as mean ± SEM of three independent experiments. The asterisk marked the statistically significant difference.

(a)

(b)

(c)

(d)