Paracrine Regulation of Steroidogenesis in Theca Cells by Granulosa Cells Derived from Mouse Preantral Follicles

Figure 3

Effect of granulosa cells on mRNA expression of genes encoding steroidogenic enzymes (Star, Cyp11a1, Cyp17a1, and Hsd3b2) in TCs. After TCs were cultured up to 72 h, the expressions of Star, Cyp11a1, Cyp17a1, and Hsd3b2 were measured by quantitative real-time PCR (GAPDH as internal control). Expressions of all these genes were increased in the cocultured model compared with TCs cultured alone. Data were the mean ± SEM of three independent experiments where all the samples were repeated in thrice. The asterisk showed the statistically significant difference. .

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