Isolation and Characterization of Hydrocarbon-Degrading Yeast Strains  from Petroleum Contaminated Industrial Wastewater

<div>Phylogenetic dendogram data showing the taxonomic location of strains HC1 and HC4. (a) Neighbour-joining phylogenetic dendogram based on sequences of the D1/D2 domain of the 26S rRNA gene of HC1 and related taxa. Bootstrap values are given at the nodes. Scale bar represents the substitution percentage.<i> Saccharomyces cerevisiae</i> NRRL Y-1263<svg height="11.8878pt" id="M7" style="vertical-align:-0.04979992pt" version="1.1" viewbox="-0.0498162 -11.838 12.5974 11.8878" width="12.5974pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M412 140C382 77 369 73 315 73H129L270 222C362 320 402 379 402 466C402 571 322 635 234 635C177 635 130 609 99 576L42 495L64 475C90 514 133 568 201 568C274 568 318 519 318 435C318 349 255 267 193 193C144 135 87 78 32 23V0H405C417 45 427 89 440 131L412 140Z" id="g113-51"></path><glyph.data ascent="3473" descent="-2876" horiz-adv-x="480" vert-adv-y="480"></glyph.data></g><g transform="matrix(.0091,0,0,-0.0091,6.24,-5.741)"><path d="M601 496C596 555 591 627 591 675H567C551 656 544 650 516 650H120C91 650 79 651 64 675H41C38 619 34 555 29 492H63C75 537 83 566 96 582C110 604 128 612 207 612H272V126C272 46 264 38 170 33V0H464V33C368 38 359 48 359 126V612H430C502 612 520 605 535 582C547 566 556 541 567 492L601 496Z" id="g58-82"></path><glyph.data ascent="1007" descent="-369" horiz-adv-x="628" vert-adv-y="628"></glyph.data></g></svg> was used as outgroup. GenBank accession numbers follow species name in parenthesis. (b) Phylogenetic dendogram obtained by neighbour-joining analysis of the 5.8S-ITS sequence of HC4 and related taxa. Bootstrap values, determined from 100 replicates, are shown at branch nodes. Scale bar represents the substitution percentage. The outgroup we used was<i> Tsuchiyaea wingfieldii</i> CBS 711<svg height="12.0444pt" id="M8" style="vertical-align:-0.2063999pt" version="1.1" viewbox="-0.0498162 -11.838 12.5974 12.0444" width="12.5974pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M249 635C141 635 70 555 70 471C70 401 114 353 179 316C143 294 106 267 90 252C68 231 45 202 45 157C45 50 130 -12 237 -12C322 -12 435 52 435 169C435 256 372 304 303 343C349 374 375 398 383 407C401 429 411 458 411 487C411 569 344 635 249 635ZM238 603C285 603 337 567 337 482C337 422 310 385 276 358C205 393 145 426 145 500C145 552 179 603 238 603ZM248 20C183 20 125 70 125 163C125 218 158 268 206 300C284 261 355 217 355 143C355 66 308 20 248 20Z" id="g113-57"></path><glyph.data ascent="3473" descent="-2876" horiz-adv-x="480" vert-adv-y="480"></glyph.data></g><g transform="matrix(.0091,0,0,-0.0091,6.24,-5.741)"><path d="M601 496C596 555 591 627 591 675H567C551 656 544 650 516 650H120C91 650 79 651 64 675H41C38 619 34 555 29 492H63C75 537 83 566 96 582C110 604 128 612 207 612H272V126C272 46 264 38 170 33V0H464V33C368 38 359 48 359 126V612H430C502 612 520 605 535 582C547 566 556 541 567 492L601 496Z" id="g58-82"></path><glyph.data ascent="1007" descent="-369" horiz-adv-x="628" vert-adv-y="628"></glyph.data></g></svg>. GenBank accession numbers follow species name in parenthesis.</div>

BioMed Research International

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Figure 2

Figure 2: Isolation and Characterization of Hydrocarbon-Degrading Yeast Strains  from Petroleum Contaminated Industrial Wastewater 