Development of Microsatellite Markers Derived from Expressed Sequence Tags of Polyporales for Genetic Diversity Analysis of Endangered Polyporus umbellatus

Table 7

Twenty-one EST-SSR primers selected out of 30 were evaluated for their amplifications on 13 Polyporus umbellatus accessions.

Primer code	Observed number of alleles (na)	Effective number of alleles (ne)	Polymorphism information content (PIC)	Observed heterozygosity (Ho)	Expected heterozygosity (He)	Nei’s gene diversity	Shannon information index ()
GL01	2	2.00	0.38	1.00	0.53	0.50	0.69
GL02	2	1.72	0.33	0.60	0.42	0.42	0.61
GL03	2	1.89	0.36	0.77	0.47	0.47	0.67
GL09	2	1.11	0.09	0.10	0.10	0.10	0.20
GL10	2	1.15	0.12	0.14	0.14	0.13	0.26
GL11	2	1.16	0.13	0.00	0.15	0.14	0.27
GL23	2	2.00	0.38	0.54	0.52	0.50	0.69
PS02	2	1.94	0.37	0.67	0.49	0.49	0.68
PS05	3	2.04	0.46	0.69	0.51	0.51	0.88
PS06	2	1.88	0.36	0.75	0.47	0.47	0.66
PS07	2	1.97	0.37	0.44	0.49	0.49	0.69
PS08	3	2.32	0.48	0.83	0.57	0.57	0.92
PS09	2	1.00	0.36	0.67	0.44	0.44	0.64
PS10	2	1.80	0.37	0.83	0.49	0.49	0.68
FP11	2	1.00	0.37	0.83	0.50	0.50	0.69
FP12	2	1.98	0.37	0.85	0.50	0.49	0.68
FP13	2	2.00	0.38	1.00	0.50	0.5	0.69
FP14	2	1.98	0.37	0.73	0.49	0.50	0.69
FP15	2	1.54	0.29	0.27	0.35	0.35	0.54
FP16	2	1.75	0.34	0.36	0.43	0.43	0.62
FP17	2	1.82	0.35	0.54	0.45	0.45	0.65
Mean	2	1.74	0.33	0.52	0.41	0.39	0.57