Skin-Derived Precursor Cells Promote Angiogenesis and Stimulate Proliferation of Endogenous Neural Stem Cells after Cerebral Infarction

Figure 4

SKPs increase SVZ and IBZ neurogenesis and neuronal progenitor migration to the ischemic lesion 14 days after MCAO. Immunostaining of neural stem cells markers and colocalization of BrdU in (a) SVZ and (b) IBZ of the ischemic rat brain. Brain sections were immunostained for both ((a)(I); (a)(III); (b)(I); (b)(III)) BrdU (green) and nestin (red) or for ((a)(II); (a)(IV); (b)(II); (b)(IV)) βIII-tubulin in SKPs group (top) and PBS (bottom). ((i), (ii)) Higher magnification of indicated by the white box. Arrowhead shows BrdU positive cells that were colocalized with nestin. Scale bars, 10 μm. (c) The pattern of implanted SKPs after cerebral brain ischemia. SVZ and IBZ were indicated by the black box, shadow area, and infracted zone. Quantitative analysis of (d) BrdU, (e) βIII-tubulin, and (f) nestin positive cells in the SVZ and IBZ ( for control and SKPs group). , versus control.

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