IFN-CSP Inhibiting Hepatitis B Virus in HepG2.2.15 Cells Involves JAK-STAT Signal Pathway

Figure 1

IFN-CSP treatment suppressed HBV antigens secretion and HBV-DNA replication in HepG2.2.15 cells. HepG2.2.15 cells were cultured in the presence of IFNα2b and IFN-CSP at various concentrations for 9 days and the cell survival rates were measured by MTT method (a). Hepatitis B surface antigen (HBsAg; b) and hepatitis B e antigen (HBeAg; c) in the culture supernatants were analyzed by enzyme-linked immunosorbent assay (ELISA). HBV-DNA (d) in the culture supernatants was measured by real-time quantitative PCR. Data represent the mean ± SEM of three experiments. as compared with the no-drug control group.

(a)

(b)

(c)

(d)