cspA Influences Biofilm Formation and Drug Resistance in Pathogenic Fungus Aspergillus fumigatus

Figure 3

cspA gene disruption changes the morphology of A. fumigatus. (a) Conidia of A. fumigatus 293T wild type (WT), ΔcspA, and cspAC were inoculated and cultured in 37°C. 10⁵ conidia in 10 μL were spotted in the center of PDA plates and cultured for 4 d. The colonial morphology was observed when cultured for 72 h. (b) The coverslips were also stained by lactophenol cotton-blue and observed by light microscope immediately. The data exhibited here are the mean ± SE ( for every experiment). The statistical differences existing between mutants and WT are analyzed with -test. ^*. (c) The coverslips covered on PDA cubes with mycelia were transferred and prefixed with 2% glutaraldehyde at 4°C for 10 h and then metalized with gold and investigated by scanning electron microscope.

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