Quantification of Internalized Silica Nanoparticles via STED Microscopy

Figure 2

Enlarged sections of an A549 cell exposed to Si-25-FD particles for 5 h. ((a), (c), and (d)) They are orthogonal sections of the area indicated in Figure 1(a). Intersecting planes pass the middle of the image (cross hair). SNPs (magenta), membrane (cyan), and nuclear lamina (yellow). (b) An intensity plot through a sample nanoparticle (yellow line) within the cell. The full width of half maximum (FWHM) was 61 ± 4 nm, which is well below the classical optical resolution limit (error: standard deviation of Gaussian fit).

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(b)

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