Quantification of Internalized Silica Nanoparticles via STED Microscopy

Figure 3

Enlarged sections of an A549 cell exposed to Si-85-FD particles for 5 h. ((a), (c), and (d)) They are orthogonal views of the area indicated in Figure 1(b). Intersecting planes pass the middle of the image (cross hair). SNPs (magenta), membrane (cyan), nuclear lamina (yellow). (b) is an intensity plot through an example nanoparticle (yellow line). The full width of half maximum (FWHM) was 81 ± 4 nm, indicating that single NPs can be detected in the cytosol (error: standard deviation of Gaussian fit).

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