Specific Proteins in Nontuberculous Mycobacteria: New Potential Tools

Figure 2

Representative 2D-PAGE of NTM cellular proteins of (a) M. nonchromogenicum type I, (b) M. peregrinum, (c) M. nonchromogenicum type II, (d) M. scrofulaceum/M. mantenii, and (e) M. gordonae. Eighty micrograms of cell proteins was isoelectrically focused in IPG strips (pH 4–7) and run on sodium dodecyl sulphate (SDS) 12.5% polyacrylamide gel. Gels were silver-stained and analysed with PDQuest 2D Analysis V8.0 (Bio-Rad, USA). The yellow and red circles were used to identify common proteins to all NTM strains and specific proteins to each NTM strain, respectively, by MS-based techniques.

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