Specific Proteins in Nontuberculous Mycobacteria: New Potential Tools
Table 4
Proteins identified by sequencing in NTM strains.
Protein
Name
Function
Gene name
Peptides ID
Global scorea
Sequence coverage %
Reference sequence
FCb
Proteins identified in M. nonchromogenicum type I
1
Deoxyuridine 5′-triphosphate nucleotidohydrolase
Involved in the biosynthesis of thymidylate. This enzyme is involved in nucleotide metabolism Catalytic activity: dUTP + H(2)O = dUMP + pyrophosphate
dut
2
109
11
M. gilvum
7
2
Probable 3-hydroxyl-thioester dehydratase
Unknown
htdZ
3
65
23
M. tuberculosis H37Rv
7
Proteins identified in M. nonchromogenicum type II
1
Conserved hypothetical protein
Unknown
—
4
33
27
M. tuberculosis complex
10
2
Catalase-peroxidase KATG
Multifunctional enzyme that exhibits a catalase, a broad-spectrum peroxidase, and peroxynitritase activities may play a role in the intracellular survival of mycobacteria within macrophages for the protection against reactive oxygen and nitrogen intermediates produced by phagocytic cells
katG
4
115
6
M. avium subsp. paratuberculosis K-10
0
Proteins identified in M. peregrinum
1
Mannose-binding lectin
Unknown
MSMEG3662
4
114
30
M. smegmatis
—
2
Inositol-5-monophosphate dehydrogenase
IMPDH catalyses the NAD-dependent oxidation of inosine 5′-monophosphate (IMP) to xanthosine 5′-monophosphate (XMP)
PRK05567
3
125
6
M. vanbaalenii
7
Proteins identified in M. gordonae
1
Probable cold shock protein A
Possibly involved in the acclimation to cold temperatures (the production of the protein is thought to be induced at low temperatures)
cspA
1
34
14
M. tuberculosis H3Rv
0
2
Putative mannose-specific lectin precursor
Lysine domain, found in a variety of enzymes involved in bacterial cell wall degradation. This domain may have a general peptidoglycan binding function
MAB2373
2
72
18
M. abscessus
10
3
Superoxide dismutase
Destroys toxic radicals that are normally produced within the cells Catalytic activity: 2 peroxide radicals + 2H(+) = O2 + H2O2
sodA
2
81
24
M. gordonae and M. assiaticum
0
4
Malate dehydrogenase
Involved in the conversion of malate to oxaloacetate Catalytic activity: (S)-malate + NAD+ = oxaloacetate + NADH
mdh
5
197
14
M. marinum
7
5
Luciferase-like protein
Energy production and conversion
Mmcs 0532
3
151
9
Mycobacterium sp.
—
6
F420-dependent glucose-6-phosphate dehydrogenase
Catalyses oxidation of glucose-6-phosphate to 6-phosphogluconolactone using coenzyme F420 (hydroxy-5-deazaflavin derivative) as the electron acceptor
fgd
3
148
10
M. avium (M. ulcerans, M. marinum, M. chelonae)
7
7
Hypothetical protein SKA58_12772
Predicted phosphohydrolases
SKA58 12772
1
52
3
Sphingomonas sp. SKA58
—
Common proteins to all NTM strains
1
RNA polymerase beta subunit
Catalyses the transcription of DNA into RNA using the four ribonucleoside triphosphates Catalytic activity: N nucleoside triphosphate = N diphosphate + [](N)
rpoB
2
30
72
M. tuberculosis
2
2
50S ribosomal protein L7/L12
Involved in translation mechanisms. Thought to be the binding site for several of the factors involved in protein synthesis and appears to be essential for accurate translation
rplL
4
115
35
Mycobacterium sp. (M. smegmatis and M. vanbaalenii)
2
3
Adenylate kinase
This enzyme is essential in intracellular nucleotide metabolism; in addition, it has been found to act as both a nucleoside mono- and diphosphate kinase suggesting it may have a role in RNA and DNA biosynthesis Catalytic activity: ATP + AMP = ADP + ADP
adk
1
53
6
M. leprae
7
4
Short-chain dehydrogenase/reductase SDR
Involved in the fatty acid biosynthesis pathway (first reduction step) (mycolic acid biosynthesis)
M. tuberculosis H37Rv, M. avium subsp. paratuberculosis K-10
10
7
Conserved hypothetical protein
Unknown
Rv3075c
2
65
4
M. tuberculosis H37Rv
10
8
Probable aldehyde dehydrogenase
Interconversion aldehyde and acid. Catalytic activity: an aldehyde + NAD+ + H2O = an acid + NADH
Rv0458
2
41
4
M. tuberculosis, M. bovis, M. leprae
7
9
Enolase
Catalysing the reversible conversion of 2-phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis Catalytic activity: 2-phospho-D-glycerate = phosphoenolpyruvate + H2O
eno
4
77
10
M. bovis AF2122/97
7
10
DNA polymerase III (beta chain) DNAN
DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3′ to 5′ exonuclease activity. The beta chain is required for initiation of replication once it is clamped onto DNA; it slides freely (bidirectional and ATP-independent) along duplex DNA Catalytic activity: N-deoxynucleoside triphosphate = N-diphosphate + (DNA)n
dnaN
44
11
M. tuberculosis
2
aAccording to Mascot search results, protein scores >25 are significant (). bFC: functional category. 0: virulence, detoxification, and adaptation; 1: lipid metabolism; 2: information pathways; 3: cell wall and cell processes; 4: stable RNAs; 5: insertion sequences and phages; 6: Pe/PPE; 7: intermediary metabolism and respiration; 8: unknown; 9: regulatory protein; 10: conserved hypothetical (from BCGList World-Wide Web Server http://genolist.pasteur.fr/BCGList).