Inhibition of PI3K Signalling Selectively Affects Medulloblastoma Cancer Stem Cells

Figure 1

LY294002 reduces cell proliferation and vitality of MDB cell lines by efficiently inhibiting PI3K/AKT signalling. (a) Representative images of DAOY MDB cells after 48 h of treatment with 15 μM LY294002 (LY) compared to control (DMSO). Original magnification 20x, white bar: 50 μm. (b) Bar graph reporting Trypan Blue cell counts of DAOY cells after 48 h of 15 μM LY294002 treatment. Mean of 3 experiments ± S.E.M. (c, d) Representative dot plot showing Annexin-V/PI staining of DAOY cells after 15 μM LY294002 treatment (c) and bar graph summarizing Annexin-V/PI analysis derived from 3 independent experiments ± S.E.M (d). (e) Graphs showing relative expression of the PI3K/AKT signalling components (phosphorylated at specific residues) 4EBP1, AKT, eIF4G, GSK3α, IRS1, and PDK1 as measured by RPPA. For all graphs, and .

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