Inhibition of PI3K Signalling Selectively Affects Medulloblastoma Cancer Stem Cells

Figure 2

LY294002 inhibits PI3K/AKT signalling and proliferation of MDB patients-derived primary cells. (a) Representative images of HuTuP33 primary MDB cells at different time points from LY294002 (LY) treatment (15 μM). Original magnification 20x, bar: 50 μm. (b) Western blot analysis performed on FI25 primary MDB cells at different time points from LY294002 treatment (15 μM). β-actin levels have been used as loading control and protein phosphorylation levels compared to the corresponding total proteins. Similar results have been obtained also for HuTuP33 and HuTuP49 MDB primary cells. (c, d) Representative immunofluorescence images of FI25 cells after LY294002 treatment (15 μM) showing a significant decrease of Ki67 (red) expression (c) and relative quantification (d). Original magnification 10x, bar: 50 μm. Similar images were obtained also for HuTuP33 primary MDB cells. Mean of 3 independent experiments ± S.E.M, .

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