Targeted Mutation of Nuclear Bone Morphogenetic Protein 2 Impairs Secondary Immune Response in a Mouse Model

Figure 2

mice responded normally to primary systemic infection with S. aureus. (a)–(d) Mice were infected by tail vein injection with 3 × 10⁵ CFU/g S. aureus and followed up for 3 days. (a) Percent survival ( per group). (b) Weight loss is plotted as average percent of original body weight ± SE ( per group). (c) Average liver, kidney, and spleen weights as a percentage of total body weight ± SE on day 3 after primary infection ( per group). (d) Various tissues were cultured on day 3 after primary infection to measure bacterial load, and results shown are average CFU/mL ± SE for blood samples and average CFU/g ± SE for the other tissues ( per group). (e) Mice were infected by tail vein injection with 1 × 10⁴ CFU/g S. aureus, a dose that caused no mortality. Tissues were cultured on day 8 after primary infection to measure bacterial load, and results shown are average CFU/mL ± SE for blood samples and average CFU/g ± SE for liver, spleen, kidney, and lymph node samples ( for wild type and for mutant mice).

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