Pathogenic Potential of Two Sibling Species, Anisakis simplex (s.s.) and Anisakis pegreffii (Nematoda: Anisakidae): In Vitro and In Vivo Studies
Table 2
Comparison of cumulative penetration of Anisakis simplex (s.s.) and Anisakis pegreffii in 2 different agar blocks overlaid with 3 different supernatants. Data are expressed as the average penetration rate ± standard error of five replicates.
Supernatant
Times after administration
A. simplex (s.s.)
A. pegreffii
valuea
Group A (agar block in PBS)
PBS
1 h
10.0 ± 8.1
25.0 ± 5.9
0.172
12 h
25.0 ± 12.9
41.7 ± 2.6
0.242
24 h
30.0 ± 14.1
41.7 ± 2.6
0.439
48 h
51.7 ± 12.2
50.0 ± 3.7
0.898
AGJ
1 h
10.0 ± 8.1
68.3 ± 6.1
0.000*
12 h
38.3 ± 9.0
81.7 ± 6.1
0.004*
24 h
41.7 ± 9.1
81.7 ± 6.1
0.007*
48 h
43.3 ± 10.0
83.3 ± 7.0
0.011*
RPMI-1640
1 h
8.3 ± 3.7
40.0 ± 4.9
0.001*
12 h
25.0 ± 12.4
46.7 ± 5.7
0.149
24 h
28.3 ± 11.1
48.3 ± 5.5
0.144
48 h
46.7 ± 10.1
51.7 ± 4.1
0.657
Group B (agar block in RPMI-1640)
PBS
1 h
15.0 ± 4.9
50.0 ± 8.3
0.007*
12 h
25.0 ± 7.0
55.0 ± 7.7
0.020*
24 h
58.3 ± 12.6
63.3 ± 7.7
0.744
48 h
76.7 ± 7.2
65.0 ± 6.7
0.268
AGJ
1 h
5.0 ± 2.0
33.3 ± 10.9
0.033*
12 h
31.7 ± 14.0
43.3 ± 12.5
0.552
24 h
56.7 ± 10.0
46.7 ± 12.0
0.538
48 h
75.0 ± 7.9
46.7 ± 12.0
0.044*
RPMI-1640
1 h
10.0 ± 6.1
53.3 ± 4.3
0.000*
12 h
28.3 ± 8.2
66.7 ± 8.3
0.011*
24 h
45.0 ± 13.1
71.7 ± 9.7
0.140
48 h
71.7 ± 8.6
71.7 ± 9.7
1.000
aby independent -test. *Statistically significant ( < 0.05).
