Biological Activities of Aerial Parts Extracts of Euphorbia characias

Table 5

Identification of polyphenolic compounds in E. characias leaves ethanolic extract using LC-ESI-Orbitrap-MS/MS in negative ion mode and quantification by LC-DAD.

	Putative identification	RT (min)	g/L (mean ± SD)	MW	M − H]^−	Molecular formula	MS/MS	References
1	Gallic acid	7.17	0.94 ± 0.23	170.0215	169.1195	C7H6O5	125.02	[35]
2	Catechin	14.75	0.65 ± 0.04	290.2680	289.0715	C15H13O6	245.05; 205.04; 125.02	[35]
3	Unknown	20.39	NQ		951.0734		932.70	—
4	Myricetin-hexose	23.16	0.02 ± 0.00	480.0904	479.0824	C21H19O13	317.06	[35]
5	Unknown	24.56	NQ		960.789		913.01	—
6	Myricetin-deoxyhexose	26.49	0.01 ± 0.00	464.0954	463.0873	C21H19O12	317.06	[35]
7	Quercetin-3-O-glucoside	27.52	1.94 ± 0.09	464.0954	463.0873	C21H19O12	301.07	[25]
8	Unknown	28.97	NQ		469.0516	C22H13O12	393.07	—
9	Quercetin-3-O-xyloside	29.20	2.25 ± 0.16	434.0849	433.0771	C20H17O11	301.07	[25]
10	Quercetin-3-O-arabinoside	30.18	9.54 ± 0.36	434.0849	433.0771	C20H17O11	301.07	[25]
11	Quercetin-3-O-rhamnoside	31.02	31.88 ± 2.75	448.1005	447.0924	C21H19O11	301.25	[25]
12	Di-O-caffeoylquinic acid	31.07	0.02 ± 0.00	516.0962	515.0800	C17H23O18	353.10; 191.02	[35]
13	3,3′-Dimethyl ellagic acid pentose	31.53	0.01 ± 0.00	462.0798	461.0877	C22H19O12	329.02	[36]
14	3,3′-Dimethyl ellagic acid deoxyhexose	35.70	0.01 ± 0.00	476.0954	475.0877	C22H19O12	329.02	[36]
15	Quercetin-3-(2-O-acetyl)-arabinoside	35.74	39.99 ± 2.52	476.0954	475.0877	C22H19O12	300.08	[25]
16	Acacetin glucuronide	39.84	0.50 ± 0.02	460.1005	459.0923	C22H19O11	283.27	[35]

Quantified using corresponding authentic standard; quantified as equivalent of myricetin-3-O-glucoside; quantified as equivalent of quercetin-3-O-glucoside; quantified as equivalent of chlorogenic acid; quantified as equivalent of ellagic acid; quantified as equivalent of acacetin; NQ: not quantified.