An Ash1-Like Protein MoKMT2H Null Mutant Is Delayed for Conidium Germination and Pathogenesis in Magnaporthe oryzae

Figure 3

MoKMT2H is required for conidium formation and pathogenicity on rice leaves. (a) Microscopic observation of conidium formation in the wild-type strain and ΔMoKMT2H null mutants on barley leaves at 9 and 36 h after inoculation. Bar 20 μm. AP: appressorium, IH: infectious hyphae. (b) Conidiation of P131 and ΔMoKMT2H null mutants after growth on OTA plates for 7 days. Comparison of the conidium formation rate between P131 and ΔMoKMT2H null mutants at 48 h after conidiation. Values are the mean ± SD from three biological replicates. (c) Conidia spray (left), mycelium plug (middle), and conidia droplet inoculation on rice leaves with conidia from the wild-type P131 and the ΔMoKMT2H null mutants. Typical leaves were observed 7 days after mycelium plug inoculation. Mycelium plug and conidia droplet inoculation was conducted on abraded rice leaves. Typical leaves were observed 5 days after mycelium plug inoculation. (d) Conidia spray (left) and mycelium plug (right) inoculation on barley leaves with conidia from the wild-type P131 and the ΔMoKMT2H null mutants. ☆ indicates .

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