Research Article
Improved Stability of a Model IgG3 by DoE-Based Evaluation of Buffer Formulations
Table 2
Full factorial DoE for dual buffer component (His/Arg) formulations. Detailed composition of each buffer tested in the 24 full factorial DoE.
| | Pattern | Arginine (mM) | Histidine (mM) | NaCl (mM) | pH |
| HR 1 | ++−+ | 200 | 50 | 50 | 6.5 | HR 2 | +−+− | 200 | 25 | 100 | 6.0 | HR 3 | ++−− | 200 | 50 | 50 | 6.0 | HR 4 | ++++ | 200 | 50 | 100 | 6.5 | HR 5 | −−−− | 100 | 25 | 50 | 6.0 | HR 6 | −+++ | 100 | 50 | 100 | 6.5 | HR 7 | −+−+ | 100 | 50 | 50 | 6.5 | HR 8 | −++− | 100 | 50 | 100 | 6.0 | HR 9 | −−+− | 100 | 25 | 100 | 6.0 | HR 10 | −+−− | 100 | 50 | 50 | 6.0 | HR 11 | −−−+ | 100 | 25 | 50 | 6.5 | HR 12 | +−++ | 200 | 25 | 100 | 6.5 | HR 13 | +−−+ | 200 | 25 | 50 | 6.5 | HR 14 | +++− | 200 | 50 | 100 | 6.0 | HR 15 | −−++ | 100 | 25 | 100 | 6.5 | HR 16 | +−−− | 200 | 25 | 50 | 6.0 |
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