Voltage-Dependent Inactivation of MscS Occurs Independently of the Positively Charged Residues in the Transmembrane Domain

Figure 3

Inactivation of the wild-type and mutant MscS expressed in MJF455 (ΔmscSΔmscL) cells. (a) Channel currents (upper) of the wild-type and R46N MscS at different voltages ranging from −60 to +80 mV (middle). Subsaturating negative pressure (bottom) was applied after the change in voltage. (b) The time constant of inactivation at each voltage. Time constant was faster at depolarizing voltages of +60 and +80 mV (mean ± SEM, –9).

(a)

(b)