Research Article

Beneficial Effect of Short Pretransplant Period of Hypothermic Pulsatile Perfusion of the Warm-Ischemic Kidney after Cold Storage: Experimental Study

Figure 4

The use of pulsatile hypothermic machine perfusion after cold storage is associated with decreased profibrotic cytokines. (a) Localization of TGF-β in kidney sections. Note the localization in tubules and decreased expression in sections perfused with pulsatile machine after cold storage compared with only cold storage before the autotransplant (magnification 20x). (b) Semiquantification of TGF-β immunostaining in renal cells. Results are expressed as mean ± SEM (–12 animals per group). versus control group; versus cold storage plus autotransplant group. CS: cold storage; HMP: hypothermic pulsatile machine; AT: autotransplant. Bar: 100 μm.
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