Beneficial Effect of Short Pretransplant Period of Hypothermic Pulsatile Perfusion of the Warm-Ischemic Kidney after Cold Storage: Experimental Study

Figure 5

The use of pulsatile hypothermic machine perfusion after cold storage is associated with decreased fibrosis. Localization of collagen fibers in kidney sections by staining with Sirius Red: (a) control group; (b) cold storage (CS) plus hypothermic pulsatile machine (HMP) and subsequent transplant (AT) group; (c) cold storage (CS) and subsequent transplant (AT) group. Collagen fibers are stained in red on a yellow background (magnification ×10). (d) Semiquantification of Sirius Red staining in renal sections. Data are represented as mean ± SEM (–12 animals per group). versus control group. Bar: 100 μm.

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