Identification and Validation of Reference Genes for qRT-PCR Studies of Gene Expression in Dioscorea opposita

Figure 1

Specificity of qRT-PCR amplicons. (a) Agarose gel electrophoresis showing amplification of a single product of the expected size for each candidate gene and two target genes for reference gene validation. represents DL2,000 DNA marker. (b) Dissociation curves with single peaks generated for all amplicons.

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