Identification and Validation of Reference Genes for qRT-PCR Studies of Gene Expression in Dioscorea opposita

Figure 5

Relative expression levels of PE2.1 (a) and PE53 (b) during three different development stages of microtuber formation, normalized by different combinations of reference genes, as indicated. APT was found to be one of the most stable genes by both geNorm and NormFinder. The combination of TUB and UBQ was the optimal combination as selected by geNorm. The combination of APT and ACT was found to be optimal in the NormFinder analysis. The four most stable reference genes were suggested by both analyses. Standard error bars are indicated.

(a)

(b)