Amlodipine Ameliorates Ischemia-Induced Neovascularization in Diabetic Rats through Endothelial Progenitor Cell Mobilization

Figure 2

Effects of amlodipine and perindopril on EPC mobilization in diabetic rats after AMI. (a) CD133⁺VEGFR-2⁺ cell numbers as a function of time in rats after AMI treated with amlodipine, perindopril, or vehicle (control) ( per group). (b) Two-step analysis by flow cytometry to quantify CD133⁺VEGFR-2⁺ EPCs at peak (amlodipine and perindopril group at day 7, control group at day 5). EPC subset analysis was conducted on the Gate 1 region using fluorescein isothiocyanate- (FITC-) labelled antibody to CD45. The percentage of CD133⁺VEGFR-2⁺ cells was analyzed in the F2 region by two-color flow cytometry using a combination of phycoerythrin-Cy5.5- (PE-Cy5.5-) labelled monoclonal antibody to CD133 and allophycocyanin- (APC-) labelled monoclonal antibody directed to VEGFR-2. A: versus control group at the same time point, B: versus amlodipine group at peak, and C: versus control group at peak.

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