Crystal Structure of the N-Terminal RNA Recognition Motif of mRNA Decay Regulator AUF1
Table 1
Data collection and refinement statistics for .
Statistics
Se-SAD
Data collection
Wavelength ()
0.9796
Space group
P212121
Cell dimensions ()
, , ()
39.07, 39.41, 93.25
α, β, γ (°)
90, 90, 90
Resolution ()
50.00–1.70 (1.76–1.70)
Total reflections
524815
Unique reflections
16559 (1565)
(%)
9.3 (32.9)
/σ ()
42.2 (9.3)
Completeness (%)
99.9 (100.0)
Redundancy
7.1 (7.1)
Phasing
Overall figure of merit
0.493
Structure refinement
Resolution ()
26.6–1.70
Number of reflections
30748
/ (%)
16.98/20.40
Number of atoms; proteins/water
1280/263
RMS deviation
Bond lengths ()
0.006
Angles (°)
0.99
Average -factor ()
19.90
Ramachandran plot (%)
Favored region
100
Outliers
0
PDB accession code
5IM0
The numbers in parentheses are statistics from the highest resolution shell. , where is the observed intensity of individual reflection and is the average over symmetry equivalents. , where and are the observed and calculated structure factor amplitudes, respectively. was calculated from 5% of the data.
