Research Article
Expression and Purification of C-Peptide Containing Insulin Using Pichia pastoris Expression System
Table 1
Expression of recombinant human insulin in Pichia pastoris as evaluated by ELISA.
| Commercial insulin | Time (hrs) | Recombinant proinsulin | Protein conc. (µg/mL) | OD 495 | Mut+ clone | Mut clone | OD 495 | Protein conc. (µg/mL) | OD 495 | Protein conc. (µg/mL) |
| 5 | 0.695 0.031 | 0 | 0.447 0.015 | — | 0.473 0.020 | — | 10 | 0.706 0.015 | 24 | 0.523 0.010 | 8 | 0.587 0.120 | 11 | 15 | 0.776 0.015 | 72 | 0.749 0.023 | 20 | 0.0818 0.021 | 26 | 20 | 0.798 0.070 | 96 | 1.155 0.130 | 31 | 1.205 0.101 | 35 | 25 | 0.802 0.110 | 144 | 0.870 0.030 | 23 | 1.015 0.060 | 29 | 30 | 0.870 0.09 | | | | |
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Mut: methanol utilization +ve clone. Mut: methanol utilization slow phenotype.
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