Simvastatin Results in a Dose-Dependent Toxic Effect on Spiral Ganglion Neurons in an In Vitro Organotypic Culture Assay

Figure 1

Representative examples of rat SG explants stained with anti-neurofilament antibody. (a) Representative example of a rat SG explant grown for 72 h in culture only. (b) Representative example for SG treated for 72 h with simvastatin 1 μM. (c) Representative example for SG treated for 72 h with simvastatin 10 μM. (d) Representative example for SG treated for 72 h with simvastatin 100 μM. (e) Representative example for SG treated for 72 h with mevalonate 100 μM. (f) Representative example for SG treated for 72 h with mevalonate 100 μM and simvastatin 1 μM. Dash lines indicate the area of supporting cells. Scale bar 250 μm.

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