NLRP3 Activation Was Regulated by DNA Methylation Modification during Mycobacterium tuberculosis Infection

Figure 1

Mtb promoted NLRP3 activation and inflammatory cytokines expression. The THP-1 cells were plated in 10 cm dish and treated with 20 ng/mL PMA for 48 h; then the cultural media that contained PMA were replaced with fresh RPMI 1640 before infection. MOI was 10 : 1 in all experiments. NLRP3 (a) and inflammatory cytokines (b) mRNA relative expression levels were detected by quantitative real-time PCR 24 h after H37Rv infection. Data were normalized to housekeeping gene GAPDH according to the method. (c) Production of inflammatory cytokines was measured by ELISA 48 h after H37Rv infection (, , and ).

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