NLRP3 Activation Was Regulated by DNA Methylation Modification during Mycobacterium tuberculosis Infection

Figure 3

DNA methylation modification regulated NLRP3 activation in vitro. (a) The methylated (Sss I treated) or unmethylated pNLRP3-P1/P2 was transfected into HEK293T cells together with pRL, respectively, and each relative promoter activity was measured 48 h after transfection. The mRNA relative expression levels of NLRP3 (b) and IL-1β (c) in PMA stimulated THP-1 cells were detected 48 h after treatment with 100 nM DAC. (d) Supernatants of PMA stimulated THP-1 cells were collected 48 h after treatment by different concentrations of DAC, and the cell viability was analyzed using CCK-8 kit (; NS: no significant difference).

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