NLRP3 Activation Was Regulated by DNA Methylation Modification during Mycobacterium tuberculosis Infection

Figure 5

Methylation levels of NLRP3 promoter region during Mtb infection. Genomic DNA of THP-1 cells was extracted 24 h after Mtb infection. (a) Methylation status of CpG sites was measured by direct sodium bisulfate DNA sequencing as in Materials and Methods. Methylation status of −700 to −200 (b), −700 to −500 (c), and −500 to −200 (d) regions was analyzed. At least 10 clones were sequenced for each group.

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