Inhibitor of DNA Binding 1 Is Induced during Kidney Ischemia-Reperfusion and Is Critical for the Induction of Hypoxia-Inducible Factor-1α

Figure 3

HIF-1α and vimentin induction in the HK-2 cell H/R model. (a, b) Western blot analysis of HIF-1α and vimentin in HK-2 cells under hypoxia (H) and reoxygenation (R). Cells for reoxygenation experienced 24 hours of hypoxia. β-actin was examined as the loading control. A representative blot from 3 independent experiments is shown. (c, d) Quantitative RT-PCR was used for the analysis of HIF-1α and vimentin mRNA levels in HK-2 cells under H/R. Data are expressed as mean ± SD for HIF-1α and vimentin levels using β-actin mRNA as an internal control (). compared with the prehypoxia controls. HIF-1α, hypoxia-inducible factor-1 alpha; H/R, hypoxia-reoxygenation.

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