Development of Primer Pairs from Molecular Typing of Rabies Virus Variants Present in Mexico

Figure 5

Mammals specific rabies virus variants detected by nested endpoint PCR assays. (a) Detection of dog specific variant (lines 2–4) and bat specific variant (lines 5–7). Lines 2 and 5: brain negative sample; lines 3 and 6: external amplification by RT-PCR (1187 and 668 pb, resp.); lines 4 and 7: internal amplification by nested PCR. (b) Detection of vampire bat specific variant (lines 2–4) and skunk specific variant (lines 7–9). Lines 2 and 7: brain negative sample; lines 3 and 8: external amplification by RT-PCR (835 and 795 pb, resp.); lines 4 and 9: internal amplification by nested PCR; line 5: empty. Lines 1 in (a) and 1 and 6 in (b) correspond to 100 pb DNA-ladder.

(a)

(b)