Research Article
Development of Primer Pairs from Molecular Typing of Rabies Virus Variants Present in Mexico
Table 3
Primer details for host mammals specific rabies virus variants detection in Mexico.
| | Primer name | Sequence (3′ to 5′) | Sense | Rabies variant detection | Fragment size (pb) | Tm (°C) | Length (pb) |
| E | VAMPIROF | TTCAAGGTCAATAATCAGGTGGTCTCTC | F | Vampire bat | 836 | 59 | 22–49 | VAMPIRORC | AGACTGCTGTTCCTCATTCCTATTT | R | 53.8 | 833–857 | I | FBVFQ | ATTGGGCTCTAACAGGGGGCAT | F | 177 | 59.6 | 356–377 | FBVRCQ | ATAGAGCAGATTTTCGAGACAGCCCCCT | R | 62.5 | 575–602 |
| E | PERROF | TTCAAAGTCAATAATCAGGTGGTC | F | Dog | 1288 | 51.9 | 22–45 | PERRORC | AATCATCAAGCCCGTCCAAACT | R | 56.4 | 1288–1309 | I | FBPFQ | CAAGAATATGAGGCGGCTGAACT | F | 212 | 55 | 1099–1120 | PERRORC | AATCATCAAGCCCGTCCAAACT | R | 56.4 | 1288–1309 |
| E | MURCIELAGOF | GACCCTGATGATGTATGCTCTTAT | F | Bat | 668 | 51 | 196–219 | MURCIELAGORC | GTTCCTCACTCYTATTTCATCCA | R | 50.6 | 742–764 | I | FBMFQ | GCTTGACCCTGATGATGTATGCTCTTAT | F | 184 | 59 | 192–219 | FBMRCQ | TGGGCTCTAACAGGGGGTATGG | R | 58.7 | 358–379 |
| E | ZORRILLOF | ATAGAACAGATTTTTGAGACGGC | F | Skunk | 794 | 51.4 | 505–527 | ZORRILLORC | TGTCTCAGTTAGTTCCAATCATCAAGC | R | 56.9 | 1272–1298 | I | ZORRILLOF | ATAGAACAGATTTTTGAGACGGC | F | 359 | 51.4 | 506–527 | FBZRCQ | GTTCCTCACTCCTATTTCATCCA | R | 51.7 | 742–764 |
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Nested endpoint PCR and real-time RT-PCR primers designed. E: external; I: internal; F: forward; R: reverse. According to RABV strain SAD VA1 sequence.
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