Serum Amyloid a Promotes Visfatin Expression in Macrophages

Figure 5

PPAR-γ mediated SAA-induced Visfatin production. (a) RAW264.7 cells were stimulated with 50 μg/mL SAA for different times (1 h, 3 h, 6 h, and 12 h). Western blot showed that SAA significantly promoted the expression of PPAR-γ. (b) RAW264.7 cells were incubated for 12 hours with SAA alone (50 μg/mL) or combined with GW9662 (20 μM) for pretreatment or with Rosiglitazone alone (1 μM). Western blot showed the expression of changes of Visfatin expression. (c) After pretreatment with FPR2 siRNA (20 nM) for 6 h, WRW⁴ (30 mM) for 1 h, and PD98059 (20 μmol/L) for 2 h before SAA (50 μg/mL) for another 24 h, or stimulation of WKYMVm (100 nM) for 24 h, expression of PPAR-γ was measured with western blot. versus control group; versus control group; versus SAA treatment group; versus SAA treatment group. Data shown are means ± SEM from three independent experiments in duplicate.

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