HSP27 Inhibits Homocysteine-Induced Endothelial Apoptosis by Modulation of ROS Production and Mitochondrial Caspase-Dependent Apoptotic Pathway

Figure 2

Stable HSP27 overexpressing HUVEC line (Hsp27). The pEX-4 or HSP27-pEX-4 plasmid was inserted in HUVECs. After 2 weeks of G418 selection, high expression of HSP27 as compared to that in parental HUVECs was detected in cells by fluorescence microscopy, qRT-PCR, and western blot analyses. (a) Images of HUVECs, Neo, and Hsp27 cells obtained by fluorescence microscopy (4x original magnification); the green fluorescence represented GFP. (b) Real-time RT-PCR analysis of HSP27 mRNA expression in cell lines. Total RNA was prepared and quantified using specific primers. (c) HSP27 protein expression by western blotting. (d) The relative protein levels were normalized to β-actin. Data are expressed as mean ± SD of three independent experiments. compared with the Neo group.

(a)

(b)

(c)

(d)