HSP27 Inhibits Homocysteine-Induced Endothelial Apoptosis by Modulation of ROS Production and Mitochondrial Caspase-Dependent Apoptotic Pathway

Figure 4

HSP27 resisted Hcy-induced elevation in ROS and MMP depletion. (a) ROS in cells that were treated with 10 mM Hcy or were not treated were analyzed after 24 h by flow cytometry. (b) and (c) Data are expressed as mean ± SD of three independent experiments. Columns not sharing the same superscript letter differ significantly: compared with Neo+Hcy group; compared with the Neo group. (d) Cells were treated with 10 mM Hcy for 24 h or were not treated; then, they were collected and stained with Rh123 for flow cytometric analysis. The values indicated the percentage of Rh123 fluorescence on cells after the treatment.

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