Research Article

Phytochemical Composition and Antibacterial Activity of Hydroalcoholic Extracts of Pterospartum tridentatum and Mentha pulegium against Staphylococcus aureus Isolates

Table 3

Average content of polyphenols and respective retention time (Rt) and wavelengths of maximum absorption in the visible region () in M. pulegium extracts (by elution order).

PolyphenolsRt (min)UV (nm)UV-DAD/VIS bands (nm)
in 70% methanol
ClassMg⋅g−1 dry weight

p-Hydroxybenzoic acid13.46280227, 260sh, 295Hydroxybenzoic acid0.212 ± 0.004
Chlorogenic acid17.95320242, 300sh, 327Hydroxycinnamic acid0.387 ± 0.004
(+)-Catechin18.10280231, 280Flavan-3-ols0.212 ± 0.004
Caffeic acid21.32320243, 296sh, 324Hydroxycinnamic acid0.230 ± 0.001
Quercetin-3-O-rutinoside22.96370256, 266sh, 351Flavonol0.144 ± 0.003
Quercetin-3-O-glucoside23.63370255, 266sh, 348Flavonol0.140 ± 0.002
Neohesperidin24.84280283, 326Flavanone0.628 ± 0.001
Luteolin-7-O-glucoside25.31370254, 266, 349Flavone0.201 ± 0.004
Diosmin26.04370253, 268, 339Flavone0.623 ± 0.006
Rosmarinic acid26.78320249, 299sh, 330Hydroxycinnamic acid0.287 ± 0.006
Ferulic acid27.55320241, 296sh, 324Hydroxycinnamic acid0.277 ± 0.003
Apigenin32.65370235, 267sh, 341Flavone0.323 ± 0.002

Total of hydroxybenzoic acids0.212 ± 0.004
Total of hydroxycinnamic acids1.181 ± 0.014
Total of flavan-3-ols0.212 ± 0.004
Total of flavonols0.284 ± 0.005
Total of flavones1.147 ± 0.012
Total of flavanones0.628 ± 0.001
Total polyphenols identified3.664 ± 0.040

Values expressed as mean ± standard deviation of three replicates.