miR-145a-5p Promotes Myoblast Differentiation

Figure 2

Role of miR-145a-5p in myoblast differentiation. (a) The expressions of miR-145a-5p in the negative control (NC) and in the C2C12 cells transfected with synthesized miR-145a-5p inhibitors and mimics were measured by real-time qPCR. (b) Effect of miR-145-5p on myotubes formed in C2C12 cells. Scale bar: 10 μm. (c) After the C2C12 myoblasts were transfected with the synthesized miR-145a-5p inhibitors, mimics, or NC, the relative mRNA levels of MyoD and MyoG on the 8th day of differentiation were detected by real-time PCR. (d) Immunofluorescence test of MyoD and MyoD in the C2C12 myoblasts on the 8th day of differentiation. Scale bar: 100 μm. The significance between the two groups at the same time point was analyzed using Student’s -test. Results are shown as mean ± SEM. ; .

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