miR-145a-5p Promotes Myoblast Differentiation

Figure 4

The expression of the main upstream factors of the canonical Wnt signaling pathway during myoblast differentiation. (a) Relative mRNA levels of Wnt5a, LRP5, Axin2, and β-catenin at day 2 (2 d), day 4 (4 d), day 6 (6 d), and day 8 (8 d) of differentiation were detected by real-time PCR. (b-c) Relative mRNA levels of myf5 and MyHC on the 8th day of differentiation, when transfecting miR-145a-5p inhibitors, mimics, or NC into C2C12 cells. Results are shown as mean ± SEM. . Statistical analysis was performed using Student’s -test; .

(a)

(b)

(c)