Overexpression of β-Catenin Induces Cisplatin Resistance in Oral Squamous Cell Carcinoma

Figure 2

β-catenin knockdown promoted apoptosis and sensitivity to cisplatin but suppressed proliferation in SCC-25 cells. (a) Transfection efficiency of the cells was evaluated by the fluorescence microscopy (A: OX cells, B: OX-NC cells, C: LX cells, and D: LX-NC cells). (b) Gene expression of -catenin was confirmed by PCR. (c) Protein expression of -catenin was confirmed by Western blot analysis. (d) -catenin knockdown caused a significant decrease in cisplatin IC₅₀ of SCC-25 cells. (e) CCK-8 assay showed -catenin knockdown suppressed proliferation in SCC-25 cells. (f) Flow cytometry analysis showed -catenin knockdown promoted apoptosis (OX: overexpression, OX-NC: overexpression-negative control, LX: low expression, and LX-NC: low expression-negative control). ; (magnification: 100х).

(a)

(b)

(c)

(d)

(e)

(f)