BioMed Research International

Research Article

Oregano Essential Oil Improves Intestinal Morphology and Expression of Tight Junction Proteins Associated with Modulation of Selected Intestinal Bacteria and Immune Status in a Pig Model

Table 2

Species and genus specific primers used for real-time PCR.
GenePrimers (sense/antisense 5′-3′)Size (bp)Annealing temperature (°C)
Total bacteriaF: ACTCCTACGGGAGGCAGCAG
R: ATTACCGCGGCTGCTGG		17560
Lactobacillus spp.F: CACCGCTACACATGGAG
R: TGGAAGATTCCCTACTGCT		34158
Escherichia coliF: CATGCCGCGTGTATGAAGAA
R: TTTGCTCATTGACGTTACCCG		9660
Enterococcus faecalisF: CCCTTATTGTTAGTTGCCATCATT
R: ACAATGGGAAGTACAACGAGT		14461
TNF-αF: CACCACGCTCTTCTGCCTACTG
R: TTGAGACGATGATCTGAGTCCTTGG		11563
MCP-1F: GTCCTTGCCCAGCCAGATG
R: CGATGGTCTTGAAGATCACTGCT		14860
IL-1βF: AAAGGGGACTTGAAGAGAG
R: CTGCTTGAGAGGTGCTGATGT		28658
IL-6F: AAGGTGATGCCACCTCAGAC
R: TCTGCCAGTACCTCCTTGCT		15160
INF-γF: GAGCCAAATTGTCTCCTTCTAC
R: CGAAGTCATTCAGTTTCCCAG		14061
ZO-1F: GGCGCACGGCGAAGGTAATT
R: CTATCAAACTCAGGAGGCGGCACT		40560
OccludinF: GGAGTGATTCGGATTCTGTCTATGCT
R: CGCCTGGGCTGTTGGGTTGA		42360
β-actinF: CCAGGTCATCACCATCGG
R: CCGTGTTGGCGTAGAGGT		15860