Research Article
Biotransformation of Cholesterol and 16α,17α-Epoxypregnenolone and Isolation of Hydroxylase in Burkholderia cepacia SE-1
Table 1
Purification of the cholesterol 7β-hydroxylase from Burkholderia cepacia SE-1.
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Protein concentration was determined by the method of Bradford [16] with bovine serum albumin as the standard. Cholesterol 7β-hydroxylase activity was assayed by measuring the quantity of 7β-cholesterol. PAGE: polyacrylamide gel electrophoresis. |