Subtractive Cell-SELEX Selection of DNA Aptamers Binding Specifically and Selectively to Hepatocellular Carcinoma Cells with High Metastatic Potential

Figure 1

(a) The specific binding ability of aptamers library in selected rounds with HCCLM9 (target cells) and MHCC97L (subtractive cells) was analyzed by flow cytometry. With increasing rounds of enrichment, significant increases in fluorescence intensity were detected on HCCLM9 cells but not on MHCC97L and reached the peak at the ninth round. (b) Fluorescence shift was calculated using the equation , where , , and refer to the fluorescence of the selected aptamers pool, initial library, and the cell background, respectively.

(a)

(b)