A Long-Acting BMP-2 Release System Based on Poly(3-hydroxybutyrate) Nanoparticles Modified by Amphiphilic Phospholipid for Osteogenic Differentiation

Figure 5

The ALP activity of OCT-1 cells treated by various cultures within samples within 20 days, respectively. Statistically significant difference against the ALP activity of BPC-PHB NPs, , , and ; statistically significant difference against the ALP activity of Con-BMP, , , and . (). B-PHB NPs: the PHB nanoparticles loaded with pure BMP-2 without phospholipid; BPC-PHB NPs: the PHB nanoparticles loaded with BPC-PHB; PHB NPs: the PHB nanoparticles. In-BMP: free-BMP only within initial 2 days; Con-BMP: continuous free-BMP-2 within 20 days.