A Long-Acting BMP-2 Release System Based on Poly(3-hydroxybutyrate) Nanoparticles Modified by Amphiphilic Phospholipid for Osteogenic Differentiation

Figure 6

Quantitative PCR analysis of osteogenic gene markers expression by OCT-1 cells treated by various samples on day 20. The -axis represents the relative expression () normalized to the expression level of the house keeping gene β-Actin. (a) COL-1: collagen type-1; (b) Runx2: runt-related transcription factor; (c) MGP: matrix Gla-protein; (d) OCN: osteocalcin; (e) OPG: osteoprotegerin; (f) OPN: osteopontin (). B-PHB NPs: the PHB nanoparticles loaded with pure BMP-2 without phospholipid; BPC-PHB NPs: the PHB nanoparticles loaded with BPC-PHB; PHB NPs: the PHB nanoparticles. In-BMP: free-BMP only within initial 2 days; Con-BMP: continuous free-BMP-2 within 20 days. Statistically significant difference against BPC-PHB-NPs, , ; statistically significant difference against Con-BMP, , . ().

(a) COL-1

(b) Runx2

(c) MGP

(d) OCN

(e) OPG

(f) OPN