A Long-Acting BMP-2 Release System Based on Poly(3-hydroxybutyrate) Nanoparticles Modified by Amphiphilic Phospholipid for Osteogenic Differentiation

Figure 7

Immunohistochemical analysis of OCN, the OCT-1 cells on day 20 with (a) PHB NPs, (b) BPC-PHB NPs, (c) B-PHB NPs, (d) In-BMP, and (e) Con-BMP, respectively. B-PHB NPs: the PHB nanoparticles loaded with pure BMP-2 without phospholipid; BPC-PHB NPs: the PHB nanoparticles loaded with BPC-PHB; PHB NPs: the PHB nanoparticles. In-BMP: free-BMP only within initial 2 days; Con-BMP: continuous free-BMP-2 within 20 days. The bar is 40 μm.

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