A Long-Acting BMP-2 Release System Based on Poly(3-hydroxybutyrate) Nanoparticles Modified by Amphiphilic Phospholipid for Osteogenic Differentiation

Table 1

Primers used for SYBR green polymerase chain reaction (RT-PCR) in this study.

Gene markers	Primer sequences
	Forward	Reverse
COL-1	5′-GACGAAGACATCCCACCAAT-3′	5′-AGATCACGTCATCGCACAAC-3′
Runx2	5′-CCCAGATCATGTTTGAGACCT-3′	5′-CCTCGTAGATGGGCACAGT-3′
MGP	5′-CAAGAGAGGATCCGAGAACG-3′	5′-CGCTTCCTGAAGTAGCGATT-3′
OCN	5′-GTGCAGCCTTTGTGTCCAA-3′	5′-GCTCACACACCTCCCTCCT-3′
OPN	5′-ACTGATTTTCCCACGGACCT-3′	5′-TCAGGGTACTGGATGTCAGG-3′
OPG	5′-GGGGACCACAATGAACAACT-3′	5′-AGCTGATGAGAGGTTTCTTCG-3′

COL-1: collagen type-1; Runx2: runt-related transcription factor; MGP: matrix Gla-protein; OCN: osteocalcin; OPG: osteoprotegerin; OPN: osteopontin.