Optimized Biotransformation of Icariin into Icariside II by β-Glucosidase from Trichoderma viride Using Central Composite Design Method

Figure 5

The 2D contour plot and 3D response surface on conversion rate and three influential factors. (a) Temperature and pH at concentration of ICA equal to 0.65 mg/mL; (b) pH and concentration of ICA at a temperature equal to 40°C; (c) temperature and concentration of ICA at a pH equal to 4.0; (d) contour plot for temperature and pH; (e) contour plot for pH and concentration of ICA; and (f) contour plot for temperature and concentration of ICA.

(a)

(b)

(c)

(d)

(e)

(f)