Open-Porous Hydroxyapatite Scaffolds for Three-Dimensional Culture of Human Adult Liver Cells

Table 1

Gene expression of liver cell cultures.

Gene	Culture time (days)	Hyaluronic acid		Collagen I		No hydrogel
		Scaffold	No scaffold	Scaffold	No scaffold	Scaffold	No scaffold
CK19	5	288.16	95.97	263.85	192.42	164.23	148.33
	15	453.34	202.45	2063.79	278.88	391.36	145.73
	28	873.87	791.60	1064.61	309.64	309.73	274.06
vWF	5	1.39	0.84	0.50	0.58	1.18	0.28
	15	0.078	0.06	0.93	1.65	4.27	2.64
	28	0.12	0.03	0.19	0.34	3.62	0.80
CYP450 3A4	5	63.15	40.56	0.76	0.81	0.26	0.03
	15	5.35	32.39	4.11	2.14	0.11	0.02
	28	41.87	39.76	0.04	0.04	0.02	0.02
Albumin	5	0.46	0.43	0.56	0.58	0.10	0.20
	15	2.03	0.34	0.35	0.43	0.15	0.21
	28	0.27	0.42	0.16	0.12	0.08	0.12
ASMA	5	0.00	0.55	24.62	36.62	17.57	14.69
	15	0.00	10.21	4.06	18.34	6.88	3.19
	28	4.64	0.00	2.50	0.99	1.50	1.37

Cells were cultured for 5, 15, and 28 days with (w) or without (wo) hydroxyapatite scaffolds embedded in hyaluronic acid gel (HyA), collagen-1 gel (C1), or no gel (NG), and gene expression was measured by PCR for cytokeratin-19 (CK19), Von Willebrand Factor (vWF), cytochrome P450 3A4 (CYP3A4), albumin, and Alpha-Smooth Muscle Actin (ASMA). Data are given as means from 6 biological repeats, normalized against beta-actin expression, and significance () is marked by to indicate a value significantly greater than the previous time point and < to indicate a value significantly less than the previous time point.