MP Resulting in Autophagic Cell Death of Microglia through Zinc Changes against Spinal Cord Injury

Figure 6

Increasing cellular zinc inhibited NF-κβ in microglia. (a) After 12 hours of treatment, microglia were stained with FluoZin-3-AM in normal group, LPS group, and LPS + MP group. Scale bar = 50 μm. (b) The relative fluorescence intensity of zinc. Data were expressed as mean ± SD ( for each group). , versus normal group; , versus LPS group. (c) After the same disposition as described above, the membrane protein of microglia was collected and the expression of ZIP8 was detected by western blot. Relative density of ZIP8 was analyzed. Data were expressed as mean ± SEM ( for each group). (d) After the same disposition as described above, the expression of NF-κβ in microglia was observed by western blot. Data were expressed as mean ± SD ( for each group). , versus normal group; , versus LPS group.

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